Animal Cell Culture and Technology (THE BASICS (Garland by Michael Butler

By Michael Butler

This ebook is a superb source for college kids and starting clinical reaseraches. it's a strong resource of uncomplicated equipment and methods for winning laboratory mobile tradition techniques. many of the present telephone tradition tools are coated yet this insurance isn't very designated. back, very good for newcomers.

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Extra info for Animal Cell Culture and Technology (THE BASICS (Garland Science))

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And Steffe, R. (1992) Safety aspects of handling cells. In: Butler, M. and Dawson, M. (eds) Cell Culture Labfax, pp. 209–218. BIOS Scientific, Oxford. G. (1977) Sulphonated polystyrene as an optimal substratum for the adhesion and spreading of mesenchymal cells in monovalent and divalent saline solutions. J. Cell Physiol. 90:511–519. L. G. (1976) Stimulation of clonal growth of normal fibroblasts with substrata coated with basic polymers. J. Cell Biol. 71:727–734. A. G. (1981) The use of low-temperature subculturing and culture surfaces coated with basic polymers to reduce the requirement for serum macromolecules.

2. Laboratory design A cell culture laboratory should allow the sterile handling of cultured cells with a minimal level of contamination. The growth rate of bacterial or fungal cells is usually so much greater than that of mammalian cells that no level of contamination can be tolerated in an animal cell culture. However, it is impractical to design a laboratory that is totally free of potentially contaminating microorganisms. The laboratory is generally designed to minimize the risk of contamination for an experienced worker.

7). 3 ml per well and are suitable for cloning or replicate assays. 8. These are lightweight and normally have an adjustable volume setting. The pipettors are designed for use with sterile pipette tips. 9). For some applications porous inserts for multi-well plates are available. 4–8 µm). Cells can grow over the membrane provided. This may be useful to study cell polarity as access is provided to the basolateral and apical sides of the cell layer. 6 Tissue culture flasks (T-flasks). Courtesy of Corning Catalogue The Petri dishes and T-flasks can accommodate cultures of 2–100 ml and are suitable for both anchorage-dependent and suspension cells.

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