Brassinosteroids. A New Class of Plant Hormones by V. A. Khripach, V. N. Zhabinskii, A. E. de Groot

By V. A. Khripach, V. N. Zhabinskii, A. E. de Groot

Within the monograph, the result of medical investigations achieved through researchers of the Institute of Bioorganic Chemistry of tne nationwide Academy of Sciences of Belarus (Minsk, Belarus) and the dept of natural Chemistry of the Wageningen Agricultural college (Wageningen, The Netherlands) are offered. The ebook covers buildings, type, isolation, spectroscopic selection, biosynthesis, metabolism, physiological mode of motion, sensible functions in agriculture, and so on. of a brand new classification of plant hormones - brassinosteroids.

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A double bond in the side chain only causes minor changes in the fragmentation pattern. C. X-RAY ANALYSIS The role X-ray analysis had in the beginning of studies on BS is different from its role now. Only X-ray analysis could provide reliable and convincing information about the structure of brassinolide and congeners. After that, the data obtained by NMR, mass, and IR spectroscopy formed the basis for the establishment of structures of related compounds. Now the use of X-ray analysis for BS is important just for the elucidation of subtle details of their structure and for structural elucidation of synthetic intermediates.

Among these the Ikb mutant is considered to be the BS-deficient mutant in which the biosynthesis at the step before the formation of teasterone formation is blocked. An elevated level of isofiicosterol is characteristic for the mutant. This together with a drastically reduced content of sitosterol and campesterol can be considered as an argument in favor of restriction of the biosynthesis of BS in the transformation of 24methylenecholesterol into campesterol. Another BS-sensitive Ik mutant is likely to suffer from a blockade of biosynthesis of campestanol in Pisum sativum from campesterol.

For equatorial-equatorial and equatorial-axial interaction, this constant is 2-6 Hz; for diaxial interaction, it is 11-12 Hz. The signals of the methine protons, belonging to the secondary carbon atoms of the 2a,3a-diol group, are seldomly well resolved; usually they appear as two multiplets. Examination of the form of the signal (half-width of a line) allows its unequivocal assignment. A wide multiplet with a W/2 of 20-24 Hz is characteristic for an axial proton at C-2, whereas the equatorial proton at C-3 exhibits a narrow signal with a half-width of 8-10 Hz.

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