Carbohydrate Metabolism - Part E, 1st Edition by Willis A. Wood (Eds.)

By Willis A. Wood (Eds.)

The significantly acclaimed laboratory normal, Methods in Enzymology, is among the so much hugely revered guides within the box of biochemistry. due to the fact that 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper at the present time - actually a vital ebook for researchers in all fields of existence sciences

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B. Wilson, J. Biol. Chem. 252, 1162 (1977). 7 M. R. Henrich, J. Biol. Chem. 239, 50 (1964). METHODS IN ENZYMOLOGY, VOL. 90 Copyright © 1982by AcademicPress. Inc. All rights of reproduction in any form reserved. 1 mg of bovine serum albumin per milliliter Procedure. The assay solution consists of 10 tzl of [14C]galactose, 20 ~1 of solution 1, 50 tzl of solution 2, and 20 tzl of the sample to be assayed. 5 by 15 cm strip of D E A E paper. The strip is eluted with water by descending chromatography until the front is 2 cm from the bottom.

Chem. 255, 4673 (1980). [4] H e x o k i n a s e from Ascaris suum Muscle By S c o T t C. S u p o w l t and BEY G. HARRIS Hexose + ATP ~ hexose 6-phosphate + ADP Assay Method Principle. The hexokinase' reaction is monitored by coupling to N A D P H formation (followed by absorbance at 340 nm) with glucose-6phosphate dehydrogenase. 1. D. DiPietro and S. Weinhouse,J. Biol. Chem. 235, 2542 (1960). METHODS IN ENZYMOLOGY, VOL. 90 Copyright © 1982 by Academic Press, Inc. All rights of reproduction in any form reserved.

Chem. 241, 3546 (1966). D. Purich, H. Fromm, and F. Rudolph, Adv. Enzymol. 39, 249 (1973). 9 K. Danenberg and W. W. Cleland, Biochemistry 14, 28 (1975). [5] Glucokinase from Streptococcus mutans By E. VICTORIA PORTER and BRUCE M. 2) that is synthesized during growth on a variety of substrates. The e n z y m e p r o v e d to be significantly more stable than the glucokinase described in another strain ofS. mutans. 1 A method for purifying the e n z y m e to homogeneity from S. mutans OMZ70 and a characterization of this e n z y m e are described here.

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