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Additional resources for Carbohydrate Metabolism - Part E, 1st Edition
B. Wilson, J. Biol. Chem. 252, 1162 (1977). 7 M. R. Henrich, J. Biol. Chem. 239, 50 (1964). METHODS IN ENZYMOLOGY, VOL. 90 Copyright © 1982by AcademicPress. Inc. All rights of reproduction in any form reserved. 1 mg of bovine serum albumin per milliliter Procedure. The assay solution consists of 10 tzl of [14C]galactose, 20 ~1 of solution 1, 50 tzl of solution 2, and 20 tzl of the sample to be assayed. 5 by 15 cm strip of D E A E paper. The strip is eluted with water by descending chromatography until the front is 2 cm from the bottom.
Chem. 255, 4673 (1980).  H e x o k i n a s e from Ascaris suum Muscle By S c o T t C. S u p o w l t and BEY G. HARRIS Hexose + ATP ~ hexose 6-phosphate + ADP Assay Method Principle. The hexokinase' reaction is monitored by coupling to N A D P H formation (followed by absorbance at 340 nm) with glucose-6phosphate dehydrogenase. 1. D. DiPietro and S. Weinhouse,J. Biol. Chem. 235, 2542 (1960). METHODS IN ENZYMOLOGY, VOL. 90 Copyright © 1982 by Academic Press, Inc. All rights of reproduction in any form reserved.
Chem. 241, 3546 (1966). D. Purich, H. Fromm, and F. Rudolph, Adv. Enzymol. 39, 249 (1973). 9 K. Danenberg and W. W. Cleland, Biochemistry 14, 28 (1975).  Glucokinase from Streptococcus mutans By E. VICTORIA PORTER and BRUCE M. 2) that is synthesized during growth on a variety of substrates. The e n z y m e p r o v e d to be significantly more stable than the glucokinase described in another strain ofS. mutans. 1 A method for purifying the e n z y m e to homogeneity from S. mutans OMZ70 and a characterization of this e n z y m e are described here.