Genetic Techniques for Biological Research: A Case Study by Corinne A. Michels

By Corinne A. Michels

Molecular Genetic research is a complicated textbook to educate the idea and perform of molecular genetic research to senior undergraduates and graduates learning genetics, molecular biology and cellphone biology.

This ebook makes use of a case learn strategy, with the yeast Saccharomyces because the version genetic organism, to give an explanation for the idea and perform of molecular genetic research. It offers sufficient details so readers might be in a position to observe the method of their very own examine undertaking.

Show description

Read or Download Genetic Techniques for Biological Research: A Case Study Approach PDF

Best methodology & statistics books

Experimental Design and Data Analysis for Biologists

This crucial textbook is designed for college kids or researchers in biology who have to layout experiments, sampling courses, or learn ensuing facts. The textual content starts off with a revision of estimation and speculation trying out equipment, prior to advancing to the research of linear and generalized linear versions.

Lipid-Mediated Signaling (Methods in Signal Transduction Series), 1st Edition

Because the hugely expected replace to Lipid moment Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a present and accomplished evaluate of study equipment utilized in lipid-mediated sign transduction. Pioneering specialists offer a much-needed distillation of a decade’s worthy of advances in examine recommendations which are pertinent in realizing how lipid-mediated sign transduction ties to pathologic issues.

Studying Children in Context: Theories, Methods, and Ethics

The artwork and technological know-how of doing qualitative examine regarding youngsters is the topic of this publication. Elizabeth Graue and Daniel Walsh talk about the study technique, dealing succinctly with ordinary matters yet emphasizing the place paintings with young children offers its personal specific demanding situations. half One seems to be around the study firm, conceptualizing it as an holistic task.

Knowing Differently: Arts-Based and Collaborative Research Methods

This quantity explores the experiential study tools that permit researchers to entry their very own and their individuals' figuring out in richer methods. It includes chapters on cutting edge equipment of study and research utilizing literary kinds, functionality and visible arts, and during collaborative and interdisciplinary inquiry.

Additional info for Genetic Techniques for Biological Research: A Case Study Approach

Example text

Cartledge (1991) Separation of yeast organelles. In The Yeasts, 2nd edition, Vol. H. S. Harrison, editors. Academic Press, London, pp. 121-174. S. Shah, A. Brachat, P. R. Pringle (1998) Additional modules for versatile and economical PCRbased gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953-961. T. R. W. A. -H. Lin (1996) Strategies for ProteinPurificationandCharacterization: A LaboratoryCourse Manual. Cold Spring Harbor Press, New York. , F. Chang, N. R. Cross (1993) Negative regulation of FAR1 at the Start of the yeast cell cycle.

For this, the cell extract must be prepared under nondenaturing conditions. For example, if one's genetic analysis indicates that proteins X and Y form a complex, then protein Y should CO-IPwith protein X when anti-protein X antibody is used. Western analysis of the pelleted sample should detect protein X and, if the two proteins coIP, should also be able to detect protein Y when anti-protein Y antibody is used as the probe. METAL CHELATE AFFlNITY PURIFICATION Many natural proteins have metal binding sites for ions such as Ni2+ and Zn2+.

Thus, researchers often use Sepharose beads bound with a mixture of proteins A and G in order to achieve highaffinity binding to a broad range of antibodies that they might be using in different experiments. An antibody specific to the protein of interest is added to the cell extract (prepared under the appropriate conditions) and allowed to bind. Protein A/Gbound Sepharose beads are added and incubated to allow binding of the antibodyprotein complex to the beads. The sample is then analyzed by Western analysis.

Download PDF sample

Rated 4.75 of 5 – based on 19 votes