Lipid-Mediated Signaling (Methods in Signal Transduction by Eric J. Murphy, Thad A. Rosenberger

By Eric J. Murphy, Thad A. Rosenberger

Because the hugely expected replace to Lipid moment Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a present and complete evaluation of analysis equipment utilized in lipid-mediated sign transduction. Pioneering specialists supply a much-needed distillation of a decade’s worthy of advances in learn strategies which are pertinent in realizing how lipid-mediated sign transduction ties to pathologic issues. half I describes equipment used to spot actions of and assay the expression of other enzymes eager about lipid-mediated signaling. half II highlights using mass spectrometry to check the lipid content material in a variety of structures. half III includes chapters dedicated to suggestions used to figure out the position of lipids within the activation of gene transcription. half IV rounds out the book’s stable assurance through targeting tools used to evaluate fatty acid uptake and metabolism. Lipid-Mediating Signaling is an unequalled reference for this swiftly increasing, but resource-limited box, and it's a precious source for for mobilephone biologists, biochemists, and pharmacologists, in addition to for researchers learning signaling transduction.

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Lipid-Mediated Signaling (Methods in Signal Transduction Series), 1st Edition

Because the hugely expected replace to Lipid moment Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a present and finished review of study tools utilized in lipid-mediated sign transduction. Pioneering specialists offer a much-needed distillation of a decade’s worthy of advances in study suggestions which are pertinent in figuring out how lipid-mediated sign transduction ties to pathologic issues.

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2 Radiometric PLC Assays .................................................................... 3 Assay of PtdIns-Specific PLC ............................................................. 46 Acknowledgments.................................................................................................... 47 Appendix: Commonly Used Reagents and Buffers ................................................. 47 List of Abbreviations................................................................................................

C. et al. (1999) Group-specific assays that distinguish between the four major types of mammalian phospholipase A2. Anal. Biochem. 269:278–288. © 2010 by Taylor and Francis Group, LLC 2 Methods of Measuring the Activity and Expression of Phospholipases C Ryan A. Frieler, John A. Watt, and Thad A. 3 Characteristic Functional Unit Structures Found in the PtdIns-Specific PLC Family ............................................................... 4 Activation and Regulation of PtdIns-Specific PLC ............................

Similarly, PtdCho-specific PLC hydrolyzes PtdCho, generating diacylglycerol and phosphocholine. The diacylglycerol produced from both reactions can activate protein kinase C (PKC), while the Ins(1,4,5)P3 can act as a calcium-mobilizing second messenger, inducing the rapid release of intracellular calcium. PtdIns-specific PLC are well studied, and the different isoforms have been identified and cloned. Thorough reviews describing the PtdIns-specific PLC are available [2,7,8]. PtdCho-specific PLC have also been identified and are thought to have similar roles in cellular signaling, although the characterization of this PLC class is not as well defined.

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