By Yao-Shan Fan
Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step by step type the major FISH ideas and people molecular applied sciences past FISH on hand for diagnostic prone in genetics and oncology. The tools comprise labeling FISH probes for DNA and RNA ambitions, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There also are suggestions for multicolor fiber FISH, multi-telomere FISH, prenatal prognosis utilizing maternal blood, and preimplantation prognosis. Oncological tools comprise simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast melanoma, and CAC/PAC for melanoma cytogenetics.
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Extra resources for Molecular Cytogenetics. Protocols and Applications
Biol. 51, 159–167. 35. Carter, N. , Ferguson-Smith, M. , Perryman, M. , et al. (1992) Reverse chromosome painting: A method for the rapid analysis of aberrant chromosomes in clinical cytogenetics. J. Med. Genet. 29, 299–307. 36. , Willat, L. , Ponder, B. , and Carter, N. P. (1993) Chromatid contamination can impair the purity of flow sorted metaphase chromosomes. Cytometry 14, 97–101. 37. Guan, X. , Meltzer, P. , and Trent, J. M. (1994) Rapid generation of whole chromosome painting probes (WCPs) by chromosome microdissection.
Genomics 13, 718–725. 39. , and Berger, R. (1989) Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomes. Hum. Genet. 81, 358–362. 40. Kelly, R. , Cozzarelli, N. , Deutscher, M. , Lehman, I. , and Kornberg, A. (1970) Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. J. Biol. Chem. 245, 39–45. 41. Feinberg, A. P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
10. Landegent, J. , Baan, R. , Hoeijmakers, J. , and Van Der Ploeg, M. (1984) 2-Acetylaminofluorene-modified probes for the indirect hybridocytochemical detection of specific nucleic acid sequences. Exp. Cell Res. 153, 61–72. 11. Nederlof, P. , et al. (1989) Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences. Cytometry 10, 20–27. 2] 38 7/10/2002, 12:58 PM Labeling FISH Probes for DNA Targets 39 12. Nederlof, P. , et al. (1990) Multiple fluorescence in situ hybridization.