Nuclear pre-mRNA Processing in Plants by Y. Ru, B. -B. Wang, V. Brendel (auth.), Anireddy S. N.

By Y. Ru, B. -B. Wang, V. Brendel (auth.), Anireddy S. N. Reddy, Maxim Golovkin (eds.)

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Figure 6 illustrates the observed abundance of alternatively spliced genes (transcript clusters) as a function of the number of transcript sequences analyzed with PASA, comparing rice and Arabidopsis to human and mouse. It is clear that at this time we are approaching saturation of transcript coverage and that the prevalence of AS in Arabidopsis and rice is significantly less than that observed in vertebrates. Time will tell whether the lesser prevalence of AS is observed across other plants, since many other plant genomes are being sequenced and relevant EST resources are available or expected to accumulate.

Time will tell whether the lesser prevalence of AS is observed across other plants, since many other plant genomes are being sequenced and relevant EST resources are available or expected to accumulate. The most striking difference between AS observed between plants and animals is the types of variations encountered (Kim et al. 2007a; Nagasaki et al. 2005). Nagasaki et al. (2005) used the bit-encoding scheme described above for identifying ASTI units across six eukaryotes, including human, mouse, fly (Drosophila), worm (C.

40 43 44 48 53 54 54 55 Abstract Intron sequences in nuclear pre-mRNAs are excised with either the major U2 snRNA-dependent spliceosomal pathway or the minor U12 snRNAdependent spliceosomal pathway that exist in most eukaryotic organisms. While the predominant dinucleotides bordering each of these types of introns and the catalytic mechanism used in their excision are conserved in plants and animals, several features aiding in the recognition of plant introns are distinct from those in animals and yeast.

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