On Drawing Trees and Nature. A Classic Victorian Manual with by J.D. Harding

By J.D. Harding

Классическое пособие по рисованию природы художника викторианской эпохи Джеймса Даффилда Хардинга (1798-1863) с репринтами эскизов, каждый из которых сопровождается рядом уроков, подчеркивающих и практические и теоретические аспекты.

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Lynntech, Inc. proposes a technology, which uses nanoscale iron particles to generate dissolved hydrogen as the electron donor for the indigenous anaerobic microbes in the subsurface environment so that the natural biodegradation can be sufficient to remove perchlorate to a safe level. The nanoscale iron particles due to its size can reach pores easily, supply electrons to the anaerobic microbes in regions otherwise unreachable, and then are retrieved by pumping. This technology requires no construction of bioreactors specifically designed for the perchlorate removal but utilizes the subsurface environment as a naturally occurring anaerobic reactor so that the treatment cost will be drastically reduced.

Exposure to arsenite in drinking water has been associated with the development of skin cancer, as well as internal cancers of the 38 Drinking Water lung, liver, and bladder. Chromate has been shown to be mutagenic in a variety of test systems; in contrast, arsenite and arsenate have generally tested negative for mutagenesis. However, there is evidence that arsenic is clastogenic and it appears to enhance the mutagenic activity of other agents in co-exposures. During the previous grant period, we demonstrated that chromate induces oxidative damage to DNA in a process that involves intracellular glutathione(GSH)-mediated reduction of chromate (CrVI).

Sensitivity of this system will be studied both by semi- quantitative reverse transcription polymerase chain reaction (rtPCR) to measure whole tissue alterations in LIFR levels in response to Dox in the drinking water and by utilizing a beta-galactosidase reporter gene incorporated in the targeting construct which is also switched off in the presence of Dox. Beta-galactosidase will be visualized in situ by X-gal staining to analyze localized effects of Dox administration. Effects of Dox and preliminary analysis of biological consequences of adult LIFR ablation will be assessed in bone, the central nervous system, skeletal and cardiac muscle, lung, liver, pancreas, spleen and kidney.

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