Plant-Bacteria Interactions. Strategies and Techniques to by Christopher A. Cullis.

By Christopher A. Cullis.

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It may be the case that a bacterial culture exhibits a PGPR trait, but after a time does not because of these phase variations [104]. After the screening process, the PGPR potential shown in vitro should be tested to ensure the same effect occurs in the plant. Root colonization is a necessary requirement for the bacteria to exert its effect. PGPR inoculation in distinct plant species sometimes produces erratic results [105]; however, the factors leading to failure are unclear. The competitive interactions in the rhizosphere are not well known.

1990) Carbon economy, in The Rhizosphere (ed. M. Lynch), John Wiley & Sons, Ltd, pp. 59–97. M. L. V. (1995) Planta, 196, 788–795. R. Delhaize, E. J. (1995) Planta, 196, 103–110. , Sugimoto, A. and Kimura, M. (2004) FEMS Microbiology Ecology, 48, 179–186. 21 Torsvik, V. (1980) Soil Biology & Biochemistry, 12 (1), 15–21. 22 Ogram, A. (2000) Soil Biology & Biochemistry, 32, 1499–1504. I. (2002) Plant and Soil, 244, 9–17. G. (2002) Annals of Botany, 89, 245–253. J. E. (2004) Molecular Plant–Microbe Interactions, 17 (5), 521–531.

2 mM Co2þ, Ni2þ or vanadium(IV) (VO2þ) salts, probably reflecting their competitive binding to iron chelators and transporters to the cell. 2 mM copper(II) in the culture medium (while Cu was also accumulated by the bacterium up to 2 mg gÀ1 of dry cell biomass) [18]. This effect induced certain alterations in the FTIR spectra of both whole cells [19] and cell membranes (note that in A. brasilense membranes, in contrast to whole cells, only the Mg2þ content was increased approximately sixfold in the presence of Cu2þ) [20] as well as in electrophysical properties of the bacterial cell surface [21].

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